WebThe principle for protein adsorption to HIC media is complementary to ion exchange and size exclusion chromatography. Sample molecules containing hydrophobic and hydrophilic regions are applied to an HIC … WebColumn efficiency testing Intellectual Property Notice: The Biopharma business o f GE Healthcare was acquired by Danaher on 31 March 2024 and now operates under the Cytiva™ brand. Certain collateral materials (such as application notes, scientific posters, ... RPC and HIC media: Eluent: water Sample: 1% - 2% acetone in water For RPC and HIC:
HiPrep™ Phenyl Fast Flow (high sub) 16/10 Cytiva 28-9365-45 Sigma-Aldrich
WebHHMI’s Janelia Research Campus in Ashburn, Virginia, cracks open scientific fields by breaking through technical and intellectual barriers. Our integrated teams of lab scientists … WebThe packing requires five steps: Step Action 1 Pack the column at 1 mL/min (30 cm/h) until the bed is stabilized (approximately 20 min). 2 Increase the flow until a constant pressure of 0.5 MPa (5 bar, 72.52 psi) is reached, and pack for 5 min or until the bed height is stable. 3 Stop the flow and adjust the adapter down to the surface of the … trumpeter 1/32 sbd-3 dauntless review
TSKgel ® Phenyl-5PW HPLC Column - Sigma-Aldrich
WebColumns most suitable for packing HIC media are listed under the column packing section for each HIC medium (Chapter 3). In most cases the binding capacity of the medium and the amount of sample to be purified will determine the column size required. For a complete listing, refer to the Cytiva life sciences catalog. Column packing and efficiency Webresins in a one-meter diameter column with a 20 cm bed height extend up to 600 cm/h with a backpressure below 3 bar (0.3 MPa, 43.5 psi). Figure 2 shows the pressure/flow performance of a Fig 1. Capto™ Phenyl (high sub), Capto™ Butyl, Capto™ Octyl, and Capto™ Butyl-S expand the use of HIC at laboratory and process scales and WebCytiva™ biochemistry and molecular biology products are designed for DNA amplification, nucleic acid, and protein preparation applications. They also meet requirements for DNA analysis support in molecular diagnostics, immunodiagnostics, next-generation sequencing, and Sanger sequencing. Featured Products Extraction trumpeter 1/350 class o